Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 326-1

326-1

BIOPROSPECTING OF ANTIMICROBIAL ACTIVITIES OF Capsicum annuum L. var. annuum LEAVES EXTRACTS

Autores:

Milena Bellei Cherene (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Valdirene Moreira Gomes (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Rosana Rodrigues (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Layrana de Azevedo dos Santos (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Olney Vieira da Motta (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro) ; Gabriel Bonan Taveira (UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro)

Resumo:

The emergence of antimicrobial resistant microorganisms has prompted the search for new substances for pathogen control. Plant antimicrobial peptides (AMPs) have biotechnological potential in drug development and agriculture. This study aimed to select a Capsicum annuum phenotype for AMP isolation and evaluate their antimicrobial activities. C. annuum accessions UENF 1626, 1632, and 1381, and the cultivar Carioquinha, were used. Leaves were macerated, and extraction was performed using methanol and dichloromethane. All extracts exhibited a similar electrophoretic profile with bands at 6.5 and 3.5 kDa. Antifungal activities were initially assessed against Fusarium solani, F. lateritium, Colletotrichum lindemuthianum, Candida albicans, C. buinensis, and C. tropicalis. Fungal growth inhibition was evaluated in a liquid culture medium using extracts at 200 μg.mL^-1. Carioquinha and 1381 phenotypes demonstrated the highest antifungal potential and were tested against Saccharomyces cerevisiae, Pichia membranifaciens, and Kluyveromyces marxianus. Carioquinha and 1381 inhibited K. marxianus growth by up to 24% and 16%, respectively. A protease inhibitor CaCPin-II was isolated and characterized from Carioquinha leaf extract. To evaluate antifungal potential, K. marxianus and F. solani were selected as models. Cell viability of K. marxianus was reduced by CaCPin-II and Carioquinha extract, with IC50 values of 165.6 and 460.9 μg.mL^-1, respectively. Incubation of F. solani with Carioquinha extract at 800 μg.mL^-1 inhibited growth by 20%. Fluorescence microscopy revealed morphological changes in treated cells. Carioquinha and 1381 extracts induced morphological changes and membrane permeability in K. marxianus, and in F. solani, they caused morphological changes and increased reactive oxygen species (ROS) levels and membrane permeability, respectively. Antibacterial activities of Carioquinha extract and CaCPin-II were evaluated against Escherichia coli and Staphylococcus aureus. At concentrations up to 200 μg.mL^-1, these compounds did not inhibit bacterial growth but increased S. aureus sensitivity to antibiotics. These findings suggest further investigation of C. annuum cv. Carioquinha extract and CaCPin-II for sustainable drug and pathogen control development.

Palavras-chave:

Antimicrobial peptides, Antifungal, Biotechnological potential, Pathogen control

Agência de fomento:

FAPERJ; UENF; CAPES and CNPq